Small bowel preservation using a cavitary two-layer (University of Wisconsin solution/perfluorochemical) cold storage method
Kuroda, Y.; Sakai, T.; Suzuki, Y.; Tanioka, Y.; Matsumoto, S.; Kim, Y.; Fujita, H.; Hamano, M.; Hasegawa, Y.; Ku, Y.; Saitoh, Y.
Transplantation 61(3): 370-373
1996
ISSN/ISBN: 0041-1337 PMID: 8610342 Document Number: 460823
Preservation of the small bowel by a cavitary two-layer (University of Wisconsin solution (UW)/perfluorochemical) cold storage method was evaluated in the heterotopic rat segmental small bowel transplant model. Simple cold storage in UW was effective only for 24-hr preservation. However, the cavitary two-layer method using UW made it possible to prolong preservation time up to 48 hr. Furthermore, without oxygen bubbling, the cavitary two-layer method was not effective for 48-hr preservation. Histologic studies of grafts preserved by the cavitary two-layer method for 48 hr at 7 days after transplantation showed normal architecture of the intestinal mucosa. This study demonstrates that the oxygenation of the small bowel during preservation by the cavitary two-layer method using UW makes it possible to extend preservation time up to 48 hr in the heterotopic rat segmental small bowel transplant model.