PCR-detection of cytomegalovirus infection in oncologic and non-oncologic patients with organ and tissue transplantation
Aleksandrova, I.N.; Koshkarova, I.V.; Lyshchev, A.A.; Sitskaia, K.O.; Zubarovskaia, L.S.; Akimova, S.L.; Shabanova, L.N.; Pluzhnikova, G.F.; Rudenko, V.I.; Khanson, K.P.; Afanas'ev, B.V.
Voprosy Onkologii 42(5): 22-25
1996
ISSN/ISBN: 0507-3758 PMID: 9064897 Document Number: 457786
Cytomegalovirus (CMV) infection often causes death after organ transplantation. Therefore, adequate prevention, diagnosis and treatment of this complication are of critical importance. The experience of establishing routine PCR-detection of CMV is presented. A widely accepted PCR protocol was used for clinical purposes. The strategy included 3 steps: 1) PCR amplification of CMV-sequences from leukocytes of patients; 2) visualization of PCR-product in polyacrylamide gel; 3) verification of the identity of PCR product by its hybridization to a membrane-bound specific probe. This approach allowed to detect CMV infection in 10 out of 28 bone marrow and in 4 out of 32 kidney recipients. The administration of specific antiviral treatment led to the elimination of CMV sequences from the blood.