The effect of growth media on recovery of test microorganisms after exposure to saturated steam under pressure
Krzywicka, H.; Jakimiak, B.; Zarzycka, E.
Roczniki Panstwowego Zakladu Higieny 47(4): 439-444
1996
ISSN/ISBN: 0035-7715 PMID: 9102803 Document Number: 457712
The aim of this study was to find out which growth media give the best condition for the development of test bacteria after exposure to saturated steam under pressure. The test organisms were strains of Bacillus subtilis NCTC 3610 and Bacillus stearothermophilus NCTC 8923. The test prepared from spore suspension were exposed to saturated steam under pressure 0.2 atn-B.subtilis, and 0.7 atn-B. stearothermophilus with various length of exposure /sublethal conditions/. After the exposure the tests were placed in growth media. The obtained results show that the compositions of the medium in which spore-forming bacteria are grown after the exposure under sublethal conditions to saturated steam under pressure affects the recovery of the test organism. The media with glucose, tryptose and L-alanine provided the best conditions for growth.