Effect of Sarcocystis gigantea extract (SGE) on the replication of human immunodeficiency virus (HIV)
Drössigk, V.U.; Tietz, H.J.; Pötzsch, F.; Scholz, D.; Gantenberg, R.; Hiepe, T.
Berliner und Munchener Tierarztliche Wochenschrift 109(2): 41-45
1996
ISSN/ISBN: 0005-9366 PMID: 8720736 Document Number: 457052
Basis of this study were the previous findings regarding isolation and characterization of a Sarcocystis gigantea lectin (SGL) especially the activation of human mononuclear cells (CD3+, CD4+, CD8+, Mø, B-lymphocytes). HIV-susceptible, immortaliced cell lines (H9-, MT-4) should be investigated to examine their reactivity against SGE which contains this strong mitogen. Using lymphocyte proliferation assay a strong stimulation of noninfected CD(4+)-positive H9-cell by SGE was observed. HIV-infected H(9+)-cells showed after SGE stimulation (20-160 micrograms) an exacerbation with an optimum at day 4. The virus replication in the H(9+)-hostcells was 13 times stronger. This result could also be detected indirectly because of the higher cytotoxicity in the MT-4 cell system. Cytopathogeny was measured by MTT cellvitality assay. In parallel, the high sensitive p24 core Profile-ELISA was used to directly examine the amount of produced HIV. In case of the H(9+)-cells the virus release per cell was 5 times higher after SGE stimulation compared with control.