Three-dimensional analysis of chromosomes by a computerized fluorescence microscope system
Haraguchi, T.; Hiraoka, Y.
Nihon Rinsho. Japanese Journal of Clinical Medicine 54(9): 2574-2582
1996
ISSN/ISBN: 0047-1852 PMID: 8890595 Document Number: 456862
In order to study spatial arrangement and dynamics of chromosomes and nuclear structures, we have developed a computerized fluorescence microscope workstation. This microscope workstation is capable of rapidly acquiring three-dimensional, multiple-wavelength image data and allows accurate reconstructions of three-dimensional cellular structures stained with multiple fluorescent dyes. Using this microscope system, we have examined dynamics of chromosomes and microtubules in mammalian culture cells by combination of high-resolution analysis in fixed specimens and time-lapse analysis in living cells. A key aspect of such microscopic approaches is a capability of detecting transient events during dynamic biological processes.