Direct preconditioning of cardiac ventricular myocytes via adenosine A1 receptor and KATP channel
Liang, B.T.
American Journal of Physiology 271(5 Pt 2): H1769-H1777
1996
ISSN/ISBN: 0002-9513 PMID: 8945890 Document Number: 455243
Both adenosine receptor and ATP-sensitive K (K-ATP) channel mediate the protective effect of ischemic preconditioning in the intact heart. The objective of the present study was to determine the role of adenosine receptor and K-ATP channel as well as their interaction in simulating and mediating preconditioning of the cardiac myocyte. Cardiac ventricular myocytes cultured from chick embryos 14 days in ovo were developed as a myocyte model of preconditioning. Myocytes were preconditioned by exposing them to 5-min hypoxia, termed preconditioning hypoxia, before a second 90-min hypoxia. Preconditioning resulted in a 64 +- 3% decrease in the amount of creatine kinase released and a 66, +- 2% reduction in the percentage of myocytes (-SE, n = 11) killed. Glibenclamide or 5-hydroxydecanoic acid (5-HD), when present during the preconditioning hypoxia, blocked the preconditioning effect. Prior exposure of the myocytes to pinacidil also led to a decrease in the injury sustained during the 90-min hypoxia. The protective effect of pinacidil was blocked by glibenclamide or 5-HD, suggesting that K-ATP channel activation can mimic as well as mediate preconditioning. Adenosine receptor antagonist 8-sulfophenyltheophylline (8-SPT) blocked the protective effect of preconditioning hypoxia. Adenosine or the A-1 receptor agonist 2-chloro-N-6-cyclopentyladenosine (CCPA) can replace preconditioning hypoxia and mimic preconditioning; this effect was fully antagonized by 8-SPT, glibenclamide, or 5-HD. Adenosine A-1-receptor activation caused a glibenclamide-sensitive inhibition of the basal 45Ca influx and basal myocyte contractile amplitude, consistent with coupling of A-1 receptor to stimulation of K-ATP channel in the myocytes. The data provide direct evidence that myocyte K-ATP channel is the effector downstream from adenosine A-1 receptor in mediating the direct preconditioning of cardiac myocytes.