Deletion mapping in squamous cell carcinomas of the esophagus defines a region containing a tumor suppressor gene within a 4-centimorgan interval of the distal long arm of chromosome 9
Miura, K.; Okita, K.; Furukawa, Y.; Matsuno, S.; Nakamura, Y.
Cancer Research 55(9): 1828-1830
1995
ISSN/ISBN: 0008-5472 PMID: 7728747 Document Number: 450717
Recent studies in our laboratory indicated that inactivation of a putative tumor suppressor gene on chromosome 9q is likely to be associated with an early step of esophageal carcinogenesis. To further define a region containing the putative tumor suppressor gene, we have examined loss of heterozygosity in 37 esophageal squamous cell carcinomas using 14 microsatellite markers mapped to 9q31-q34.1. Loss of heterozygosity was observed in 30 (81%) of 37 tumors at one or more of the loci exantined, and partial or interstitial deletions at 9q31-q34.1 were detected in 13 of these tumors. On the basis of these results, we constructed a detailed deletion map and defined a commonly deleted region between the D9S262 and D9S154 loci at 9q31-q32. The genetic distance between these two loci is estimated to be approximately 4 cM.