Role of parathyroid hormone-related protein in the proliferation of vascular smooth muscle cells
Jiang, B.; Morimoto, S.; Fukuo, K.; Yasuda, O.; Chen, S.; Ogihara, T.
Mineral and Electrolyte Metabolism 21(1-3): 157-160
1995
ISSN/ISBN: 0378-0392 PMID: 7565440 Document Number: 444365
Human parathyroid hormone-related protein(1-34) (hPTHrP(1-34)) inhibited (3H)thymidine incorporation into DNA of cultured vascular smooth muscle cells (VSMC) and increased intracellular cAMP accumulation, both in a concentration-dependent manner. The potency of hPTHrP(1-34) was similar to that of human parathyroid hormone(1-34) (hPTH(1-34)), but their antagonists, hPTHrP(7-34) and hPTH(3-34), showed no effect on VSMC DNA synthesis. The inhibitory effect of PTHrP on VSMC DNA synthesis was mimicked by dibutyryl-cAMP but not by dibutyryl-cGMP. These results suggest that PTHrP, as an autocrine and/or paracrine regulator, may inhibit VSMC DNA synthesis mediated at least partly by cAMP, a second messenger which has also been proposed to mediate vascular relaxation.