Radioautography in cellular and molecular biology
Wegmann, R.; Balmain, N.; Ricard-Blum, S.; Guha, S.
Cellular and Molecular Biology 41(1): 1-20
1995
ISSN/ISBN: 0145-5680 PMID: 7773122 Document Number: 440822
A general survey is presented on the most important applications of radioactive compounds as well on fresh as on fixed tissues, with and without immunological reactions, at light and electron microscopic levels. Its goal is to show their flexibility and their extended applications, in comparison with the non-radioactive methods. But radioautography applies as well to non-cellular aspects, such as electrophoretic and chromatographic techniques, permitting a complementary and even more detailed exploration of the molecules investigated at cellular levels. A rapid information is given on the exact denomination of the radioautographic methods, on hybridization in situ and in vitro, on the different blotting techniques used for DNA, RNA and proteins, on semiquantitation and quantitation of DNA-RNA hybrids, on radioimmunodetection by fluorography and on newer filmless radioautographic systems. The organ, body and pharmacological radioautographies belong to the nuclear medicine and have been evocated briefly.