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Optimized chemiluminescent detection of DNA amplified in the exponential phase of PCR

Su, X.; Sullivan, T.F.; Bursztajn, S.; Berman, S.A.

Biotechniques 17(4): 734-736

1994

ISSN/ISBN: 0736-6205
PMID: 7833037
Document Number: 437580
We describe a procedure for quantitative detection of nucleic acids by coupling PCR with improved chemiluminescent detection techniques. After performing PCR in the exponential phase in the presence of a trace amount of digoxigenin-11-dUTP the amplified products are transferred to a positively charged nylon membrane. The membrane is cleaned with a modified method involving sodium dodecyl sulfate and ethanol washing steps to ensure low backgrounds in chemiluminescent detection. The membrane is exposed to two stacked times -ray films to increase the dynamic response in a film exposure. This sensitive and quantitative procedure is useful for molecular biology studies.

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Bizanek, R.; Manes, J.D.; Fujinari, E.M. 1996: Chemiluminescent nitrogen detection as a new technique for purity assessment of synthetic peptides separated by reversed-phase HPLC Peptide Research 9(1): 40-44
Ban, J.; Nagy, B.; Sorić, J. 1978: The influence of methylnitrosourea and adriamycin on cells in exponential and plateau-phase growth Lijecnicki Vjesnik 100(4): 234-236
Merscher, S.; Hanselmann, R.; Welter, C.; Dooley, S. 1994: Nuclear runoff transcription analysis using chemiluminescent detection Biotechniques 16(6): 1024-1026
Berger, R.; Duncan, M.R.; Berman, B. 1993: Nonradioactive gel mobility shift assay using chemiluminescent detection Biotechniques 15(4): 650 652
Nguyen, Q.; Witney, F.; Tumolo, A. 1992: A two-step hybridization method for chemiluminescent detection of single copy genes Biotechniques 13(1): 116-123
Sarkar, F.H.; Li, Y.W.; Ball, D.E.; Crissman, J.D. 1992: Comparative method for detection of RNA-PCR-amplified signals Biotechniques 12(1): 22; 24, 26
Zavattoni, M.; Percivalle, E.; Cattaneo, E.; Revello, M.G.; Torsellini, M.; Gerna, G. 2003: Optimized detection of respiratory viruses in nasopharyngeal secretions New Microbiologica 26(2): 133-140
Vonk, G.P.; Wagner, D.B. 1991: Encapsulation of europium in liposomes for use in an amplified immunoassay detection system Clinical Chemistry 37(9): 1519-1520
Dupuy, A.M.; Debarge, L.; Poulain, M.; Stéphanie Badiou; Rossi, M.; Cristol, J.P. 2009: Determination of Serum Ferritin Using Immunoturbidimetry or Chemiluminescent Detection in Comparison with Radioimmunoassay, a Compendium of a Methodological Juxtaposition Clinical Laboratory 55(5-6): 207-215
Miliukiené, V.; Dikiniené, N.; Vidziūnaité, R.; Mikalauskiené, G.; Veleckaité, A.; Mikulskis, P. 1991: The comparative evaluation of spectrophotometric and chemiluminescent detection in an immunoenzyme test of antibodies to the antigens of the bovine leukosis virus Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 7: 64-66
Pritchard, C.G.; Stefano, J.E. 1990: Amplified detection of viral nucleic acid at subattomole levels using Q beta replicase Annales de Biologie Clinique 48(7): 492-497
Tansuphasiri, U.; Suttirat, S. 2001: Simple microplate hybridization assay for detection of amplified products of Mycobacterium tuberculosis Southeast Asian Journal of Tropical Medicine and Public Health 32(4): 835-843
Yazici, H.; Kalayoglu, S.; Dalay, N. 1998: A new method for the detection of bcr/abl sequences amplified by polymerase chain reaction (PCR) following bone marrow transplantation Research Communications in Molecular Pathology and Pharmacology 101(3): 297-306
Dhahir, F.J.; Cook, D.B.; Self, C.H. 1992: Amplified enzyme-linked immunoassay of human proinsulin in serum (detection limit: 0.1 pmol/L) Clinical Chemistry 38(2): 227-232
Bernutz, C.; Kewenig, M.; Horn, K.; Pickardt, C.R. 1985: Detection of thyroid disorders by use of basal thyrotropin values determined with an optimized "sandwich" enzyme immunoassay Clinical Chemistry 31(2): 289-292
Zhdan, V.M.; Holovanova, I.A.; Filatova, V.L.; Khorosh, M.V. 2017: Medical evaluation of efficiency of optimized models for early detection and primary prevention of cardiovascular diseases Wiadomosci Lekarskie 70(3 Pt 1): 433-438
Apostol, B.L.; Black, W.C.; Reiter, P.; Miller, B.R. 1994: Use of randomly amplified polymorphic DNA amplified by polymerase chain reaction markers to estimate the number of Aedes aegypti families at oviposition sites in San Juan, Puerto Rico American Journal of Tropical Medicine and Hygiene 51(1): 89-97
Wittenbrink, M.M. 1991: Use of an amplified enzyme immunoassay for the detection of chlamydiae in a suppurative vaginal discharge from cattle with a chlamydia-induced endometritis Berliner und Munchener Tierarztliche Wochenschrift 104(4): 125-130
Kates, S.A.; Solé, N.A.; Beyermann, M.; Barany, G.; Albericio, F. 1996: Optimized preparation of deca(L-alanyl)-L-valinamide by 9-fluorenylmethyloxycarbonyl (Fmoc) solid-phase synthesis on polyethylene glycol-polystyrene (PEG-PS) graft supports, with 1,8-diazobicyclo [5.4.0]-undec-7-ene (DBU) deprotection Peptide Research 9(3): 106-113
Bronstein, I.; Fortin, J.J.; Voyta, J.C.; Juo, R.R.; Edwards, B.; Olesen, C.E.; Lijam, N.; Kricka, L.J. 1994: Chemiluminescent reporter gene assays: sensitive detection of the GUS and SEAP gene products Biotechniques 17(1): 172-4 176-7