Distinct cellular localization of mRNAs for three subtypes of prostaglandin E receptor in kidney
Sugimoto, Y.; Namba, T.; Shigemoto, R.; Negishi, M.; Ichikawa, A.; Narumiya, S.
American Journal of Physiology 266(5 Pt 2): F823-F828
1994
ISSN/ISBN: 0002-9513 PMID: 8203567 Document Number: 436858
Distribution of the mRNAs for three subtypes of prostaglandin E (PGE) receptors in the mouse kidney was investigated by in situ hybridization. The mRNA for EP-1 subtype, which is coupled to Ca-2+ mobilization, was specifically localized to the collecting ducts from the cortex to the papilla. The mRNA for EP-2 subtype, which is linked to stimulation of adenylate cyclase, was localized to the glomeruli. The mRNA for EP-3 subtype, which is coupled to inhibition of adenylate cyclase, was located densely in the tubules in the outer medulla and in the distal tubules in the cortex. These results exhibit distinct cellular localization of three subtypes of PGE receptor in the kidney and suggest that PGE-2 exerts multiple functions via these subtypes expressed in different segments of the nephron.