Antisera against three connexin43 fragments react with a 43-kD protein localised to gap junctions in myocardium and human myometrium
Kilarski, W.M.; Witczuk, B.; Pluciński, T.; Kupryszewski, G.; Roomans, G.M.; Ulmsten, U.
Folia Histochemica et Cytobiologica 32(4): 219-224
1994
ISSN/ISBN: 0239-8508 PMID: 7758614 Document Number: 435361
Gap junctions are thought to play an essential role in the control of parturition. In this study we attempted to synthesise three short peptides which match the portion of the amino acid sequence of the connexin43, protein predicted from the nucleotide sequence of cDNA clone and to produce an anti-peptide antibody which would detect gap junction protein in human myometrium. Three peptides were synthesised: (1) RKEEKLNKKEEE, (2) EJKKFKYGJEEH and (3) KNRVKGRSDPYH. Antibodies against these peptides were raised in rabbits, screened for reactivity and specificity using dot blot assays and used for immunocytochemical staining of heart and myometrial tissues.