Detection of a short segment of DNA, responsible for glucocorticoid regulation, in the 5'-flanking region on the murine metallothionein i gene
Plisov, S.I.; Merkulova, T.I.; Shkapenko, A.L.
Molekuliarnaia Biologiia 28(2): 407-412
1994
ISSN/ISBN: 0026-8984 PMID: 8183272 Document Number: 434892
Glucocorticoid responsive element (GRE) located -252 to -209 bp upstream of the transcription initiation site of mouse metallothionein I gene (mMT I) was identified in transient gene transfer experiments. This GRE, on its own, was capable of enhancing a reporter CAT gene expression in response to dexamethasone in orientation-independent manner when linked cis to a heterologous HSV TK gene promoter. The duplication of the GRE showed a synergistic effect on dexamethasone induction of the CAT gene. Nevertheless, the GRE located in its natural promoter region of mMT I gene (-330 to +70 bp) was found to provide low if any glucocorticoid induction of linked CAT gene, while showing strong cadmium regulation, comparable to the in vivo level.