Calcium in pollen-pistil interaction in Petunia hybrida Hort. I. Localization of Ca2+ ions in mature pollen grain using pyroantimonate and autoradiographic methods
Bednarska, E.; Butowt, R.
Folia Histochemica et Cytobiologica 32(4): 265-269
1994
ISSN/ISBN: 0239-8508 PMID: 7758622 Document Number: 434741
The localization of Ca-2+ in the mature pollen grain and the flow of these ions from the somatic tissues of the anther to the pollen grains has been studied using pyroantimonate and autoradiographic methods. In the pollen grain, Ca-2+ ions have been localized in the sporoderm and in the cytoplasmic vesicles of probably dictyosomal origin. Calcium ions were transported into the sporoderm together with the compounds of degenerating tapetum. The material of degenerating tapetum forms pollen coat surrounding the mature pollen grain.