Top Abstract Purchase Related
Home
>
Node
>
Subnode

Identification and biochemical characterization of secreted Wnt-1 protein from P19 embryonal carcinoma cells induced to differentiate along the neuroectodermal lineage

Papkoff, J.

Oncogene 9(1): 313-317

1994

ISSN/ISBN: 0950-9232
PMID: 8302595
Document Number: 433304
Previous studies have described Wnt-1 protein synthesized by cell lines transfected with Wnt-1 cDNA. This report presents the first identification and biochemical characterization of native Wnt-1 protein, from P19 embryonal carcinoma cells undergoing neuroectodermal differentiation. The data demonstrate that P19 cells efficiently process and secrete glycosylated Wnt-1 protein species of 40 kD and 42 kD. Similar to recombinant Wnt-1 proteins, native Wnt-1 proteins are associated with the cell surface upon secretion and can be released by suramin treatment of the cells. Non-reducing gel analysis showed that Wnt-1 proteins are secreted as monomers.

Document emailed within 1 workday
Secure & encrypted payments

St-Arnaud, R.; Craig, J.; McBurney, M.W.; Papkoff, J. 1989: The int-1 proto-oncogene is transcriptionally activated during neuroectodermal differentiation of P19 mouse embryonal carcinoma cells Oncogene 4(9): 1077-1080
Matthaei, K.I.; McCue, P.A.; Sherman, M.I. 1983: Retinoid binding protein activities in murine embryonal carcinoma cells and their differentiated derivatives Cancer Research 43(6): 2862-2867
Hara, E.; Okamoto, S.; Nakada, S.; Taya, Y.; Sekiya, S.; Oda, K. 1993: Protein phosphorylation required for the formation of E2F complexes regulates N-myc transcription during differentiation of human embryonal carcinoma cells Oncogene 8(4): 1023-1032
Sleigh, M.J.; Lockett, T.J. 1985: SV40 enhancer activation during retinoic acid-induced differentiation of F9 embryonal carcinoma cells EMBO Journal 4(13B): 3831-3837
Boothman, D.A.; Bouvard, I.; Hughes, E.N. 1989: Identification and characterization of X-ray-induced proteins in human cells Cancer Research 49(11): 2871-2878
Iurchenko, I.V.; Khromov, I.S.; Budilov, A.V.; Deev, S.M.; Sobolev, A.I. 2003: Thermostability of alkaline phosphatase of Meithermus ruber bacteria: cloning of the gene, expression in Escherichia coli cells and biochemical characterization of the recombinant protein Molekuliarnaia Biologiia 37(6): 989-998
Lesuffleur, T.; Barbat, A.; Dussaulx, E.; Zweibaum, A. 1990: Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells Cancer Research 50(19): 6334-6343
Brooks, C.G.; Georgiou, A.; Jordan, R.K. 1993: The majority of immature fetal thymocytes can be induced to proliferate to IL-2 and differentiate into cells indistinguishable from mature natural killer cells Journal of Immunology 151(12): 6645-6656
Androphy, E.J.; Hubbert, N.L.; Schiller, J.T.; Lowy, D.R. 1987: Identification of the HPV-16 E6 protein from transformed mouse cells and human cervical carcinoma cell lines EMBO Journal 6(4): 989-992
Zhang, W.; He, G.; Ma, B. 2012: Construction and expression identification of human secreted apoptosis-related protein 1 gene yeast two-hybrid bait vector Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 26(12): 1492-1496
Michel, C. 1970: Characterization of proteolytic enzymes secreted by intestinal glandular cells in 2 Annelidae Polychetae errantes Annales d'Histochimie 15(1): 19-24
Phat, V.N.; Camilleri, J.P.; Bariety, J.; Galtier, M.; Baviera, E.; Corvol, P.; Menard, J. 1981: Immunohistochemical characterization of renin-containing cells in the human juxtaglomerular apparatus during embryonal and fetal development Laboratory Investigation; a Journal of Technical Methods and Pathology 45(5): 387-390
Feickert, H.J.; Pietsch, T.; Hadam, M.R.; Mildenberger, H.; Riehm, H. 1989: Monoclonal antibody T-199 directed against human medulloblastoma: characterization of a new antigenic system expressed on neuroectodermal tumors and natural killer cells Cancer Research 49(15): 4338-4343
Miyazaki, K.; Hattori, Y.; Umenishi, F.; Yasumitsu, H.; Umeda, M. 1990: Purification and characterization of extracellular matrix-degrading metalloproteinase, matrin (pump-1), secreted from human rectal carcinoma cell line Cancer Research 50(24): 7758-7764
Grippo, J.F.; Sherman, M.I. 1990: Retinoid-binding proteins in embryonal carcinoma cells Methods in Enzymology 190: 148-155
Sherman, M.I.; Paternoster, M.L.; Taketo, M. 1983: Effects of arotinoids upon murine embryonal carcinoma cells Cancer Research 43(9): 4283-4290
Sakpichaisakul, K.; Taeranawich, P.; Nitiapinyasakul, A.; Sirisopikun, T. 2010: Identification of Sandhoff disease in a Thai family: clinical and biochemical characterization Journal of the Medical Association of Thailand 93(9): 1088-1092
Gescher, A.; Reed, D.J. 1985: Characterization of the growth inhibition induced by tumor-promoting phorbol esters and of their receptor binding in A549 human lung carcinoma cells Cancer Research 45(9): 4315-4321
Marcum, J.A.; Thompson, M.A. 1991: The amino-terminal region of a proteochondroitin core protein, secreted by aortic smooth muscle cells, shares sequence homology with the pre-propeptide region of the biglycan core protein from human bone Biochemical and Biophysical Research Communications 175(2): 706-712
Hsieh, G.C-F.; Loukas, A.; Wahl, A.M.; Bhatia, M.; Wang, Y.; Williamson, A.L.; Kehn, K.W.; Maruyama, H.; Hotez, P.J.; Leitenberg, D.; Bethony, J.; Constant, S.L. 2004: A secreted protein from the human hookworm necator americanus binds selectively to NK cells and induces IFN-gamma production Journal of Immunology 173(4): 2699-2704