Foreign genes expression in Wistar rats in vivo
Zhu, X.J.; Yao, A.Q.; Liu, B.
Zhonghua Yi Xue Za Zhi 74(3): 158-160; 191
1994
ISSN/ISBN: 0376-2491 PMID: 7922753 Document Number: 432996
pN2-LacZ or pN2-CMV-ProUK plasmid DNA was separately injected into rat quadricept muscles and beta-galactosidase or Pro-UK was detected after 2 days. The expression of foreign genes lasted for at least 3 months. pN2-LacZ plasmid was injected into the left ventricular wall of the rat, and the expression of LacZ gene was more efficient than in the quadricept muscle. pN2-LacZ was also introduced into the rat arterial wall by balloon catheter. A new method was also found to improve the efficiency of foreign gene expression in muscle by stitching medical stitch containing plasmid DNA onto the quadricept muscle.