Dengue virus. Detection and pathogeny
Castro, E.; Ayala, J.; Ramos-Castañeda, J.; Ortega, A.; Tapia-Ramírez, J.; Fernández-Tomás, C.
Archives of Medical Research 25(2): 211-214
1994
ISSN/ISBN: 0188-4409 PMID: 7919815 Document Number: 432532
Sequences from a cDNA of dengue virus type 4 were cloned into transcription vectors. These sequences included the E, NS1, NS2A, NS2B, NS3 genes. RNA transcripts produced in vitro from these plasmids were used in hybridization assays to detect dengue viral sequences. With these RNA-probes, molecules of serotype-specific dengue 4 viral RNA could be detected. Moreover, the riboprobes detected viral sequences of other serotypes in the following order of sensitivity 4>2>3>1, and might be useful to differentiate serotypes.