Direct intracellular injections for studying human myometrial gap junctions prior to labor
Ciray, H.N.; Persson, B.E.; Bäckström, T.; Roomans, G.M.; Ulmsten, U.
Acta Obstetricia et Gynecologica Scandinavica 73(2): 97-102
1994
ISSN/ISBN: 0001-6349 PMID: 8116361 Document Number: 430943
Direct intracellular microinjection of a fluorescent dye (Lucifer Yellow) was performed on ex-situ muscle strips from term pregnant women not in labor. The aim was to characterize the intracellular distribution of LY to obtain criteria for an intracellular injection in whole mounts and ultimately to study the gap junctional communication between myometrial cells in those tissues. Fifteen injections performed on biopsies from ten cases showed a well-bordered fusiform shape and were considered to be intracellular. The intercellular spread of the dye into an adjacent cell was observed in three injections (three cases). The average cell dimension was 284 +- 95 mu-m for length and 5 +- 1.5 mu-m for width (n = 17). The intracellular injection was confirmed by light microscopy of cross sections. It is concluded that limited coupling exists between myometrial cells of women prior to labor.