Radiation-induction of micronuclei in human peripheral blood lymphocytes: effect of freezing
Prosser, J.S.; Izard, B.E.; Wallace, M.A.
Cytobios 77(309): 101-106
1994
ISSN/ISBN: 0011-4529 PMID: 8020244 Document Number: 430717
Lymphocytes in peripheral blood samples from three donors were irradiated at doses of 0, 1 and 2 Gy with cobalt-60 gamma rays. Whole blood cultures were established immediately after irradiation. Cultures of Ficoll-Paque separated lymphocytes were set up immediately after irradiation, or 3 weeks after storage in liquid nitrogen at -196 degrees C. The frequency of micronuclei was measured in binucleate cytokinesis-blocked cells and the different treatment groups were compared. No clastogenic effect of the lymphocyte separation technique, or of subsequent freezing in liquid nitrogen, was observed.