Effect of gonadotropin-releasing peptide (GRP) on LH secretion of mouse pituitary in vitro
Wang, N.G.; Guan, M.Z.; Wang, D.X.; Lu, G.S.
Yao Xue Xue Bao 29(8): 581-584
1994
ISSN/ISBN: 0513-4870 PMID: 7985517 Document Number: 429978
Synthetic GRP and its analogues, GRP-NH-2, (Glu-7.9.14 Lys-6.10) GRP (6 apprx 14), (Phe-14) GRP (5 apprx 14) and (Phe-14) GRP, at the concentration of 0.05 mmol cntdot L-1 were shown to have stimulatory effect on LH secretion in cultured mouse pituitary in vitro. The luteotropin releasing activity of GRP and its analogues was estimated to be 115.4, 114.2, 140.0, 162.0 and 179.0% of the control group, respectively. Subcutaneous administration of (Phe-14) GRP on days 7 apprx 9 and 1 apprx 5 of pregnancy and (Phe-14) GRP (5 apprx 14) on days 2 apprx 4 of gestation at dosage of 1 mg cntdot d-1 (each mouse) caused fetal death in 40 apprx 60% of mice.