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A simple method for the determination of free cortisol in plasma, with normal levels in men and women, including women taking low oestrogen contraceptives

Durber, S.M.; Daly, J.R.

Clinica Chimica Acta; International Journal of Clinical Chemistry 68(1): 43-48

1976

ISSN/ISBN: 0009-8981
PMID: 1261052
DOI: 10.1016/0009-8981(76)90286-2
Document Number: 429857
A small scale equilibrium dialysis method for the measurement of the non-protein bound cortisol in plasma is described. Dialysis is carried out in PTFE cells against an isosmolar Hepes buffer, equilibration occurring within 18 hours. Inter-assay variation was found to be approximately 6%. Samples taken from normal males and females and from oestrogen-treated females were analysed and the results compared with published levels. A significant difference in both the total and free cortisol levels was found between the normals and oestrogen-treated females.

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