Lymphocyte mechanoluminescence
Orel, V.E.; Alekseev, S.B.; Reshchikov, A.M.; Komar, V.E.; Sungurov, A.I.; Grinevich, I.A.
Tsitologiia 36(8): 844-849
1994
ISSN/ISBN: 0041-3771 PMID: 7701615 Document Number: 429461
A unique installation and an original investigation method are described for studying mechanoluminescence of lymphocytes. The spectral content of mechanoluminescence of lymphocytes of human peripheral blood showed maxima of optical emission at 262, 291, 335 and 412 nm. Intraperitoneal administration of hydrocortisone in mice enhances thymocyte mechanoluminescence intensity after 2 and 3 days by 33 and 62%, resp. It is shown that Acridine orange, reacting with murine thymocytes and their nucleoids, affects intensity of their mechanoluminescence, depending on the value of concentration of the injected intercalator.