Endothelin-1 inhibits insulin-stimulated glucose uptake in isolated rat adipocytes
Chou, Y.C.; Perng, J.C.; Juan, C.C.; Jang, S.Y.; Kwok, C.F.; Chen, W.L.; Fong, J.C.; Ho, L.T.
Biochemical and Biophysical Research Communications 202(2): 688-693
1994
ISSN/ISBN: 0006-291X PMID: 8048938 Document Number: 429105
A single class of high affinity endothelin-1 (ET-1) binding sites with an apparent Kd of 350 pM and a binding capacity of 69,000 sites/cell was found in isolated rat adipocytes. Whereas ET-1 had no effect on basal glucose uptake, it inhibited insulin-stimulated glucose uptake in both a dose-and time-dependent manner. Insulin binding, however , was not altered. Thus, the present study demonstrates the presence of ET-1 receptor in isolated rat adipocytes and the inhibition of insulin-stimulated glucose uptake by ET-1 via a post-receptor mechanism.