Analysis of lipopolysaccharide (LPS) from impermeability-type drug resistant Pseudomonas aeruginosa using new, highly-sensitive LPS staining method
O'Hara, K.; Fukuda, H.; Nakahara, H.; Bryan, L.E.
Japanese Journal of Antibiotics 47(10): 1344-1347
1994
ISSN/ISBN: 0368-2781 PMID: 7528823 Document Number: 425856
The specific ladder pattern on polyacrylamide gel electrophoresis of lipopolysaccharide (LPS) extracted from Pseudomonas aeruginosa was clearly shown by using the complex methods of the PAS staining, re-periodate oxidation and then Ag-staining method. Accordingly, it was concluded that the new method was grately useful for a detail analysis of LPS changes in Gram-negative bacteria. And it was shown by this method that no changes in LPS occurred between the impermeability-type drug resistant P. aeruginosa mediated by R plasmid and a drug susceptible strain. The absence of changes indicated that the LPS of P. aeruginosa K-Ps102 had no role in the mechanism of the high drug resistance.