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Use of diethylenetriaminochloroplatinum in hybridization analysis of specific nucleic acid sequences

Kiseleva, V.I.; Turchinskiĭ, M.F.; Kolesnik, T.B.; Poverennyĭ, A.M.

Bioorganicheskaia Khimiia 20(1): 14-20

1994

ISSN/ISBN: 0132-3423
PMID: 8161359
Document Number: 424710
Monofunctional platinum compound [Pt(dien)Cl] Cl and high-affinity antibodies against DNA-[Pt(dien)Cl]Cl were suggested for non-radioactive hybridisation analysis of DNA. The simple labelling procedure was based on mixing the reagents followed by the incubation for 2 h at 60 degrees C. The sensitivity and specificity of the technique were sufficient to detect 10 fg DNA in dot-hybridisation without cross-reaction with 10-fold excess of a heterologous DNA. This technique permits genome libraries screening.

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