Crystal structure of the adenovirus DNA binding protein reveals a hook-on model for cooperative DNA binding
Tucker, P.A.; Tsernoglou, D.; Tucker, A.D.; Coenjaerts, F.E.; Leenders, H.; van der Vliet, P.C.
EMBO Journal 13(13): 2994-3002
1994
ISSN/ISBN: 0261-4189 PMID: 8039495 Document Number: 423247
The adenovirus single-stranded DNA binding protein (Ad DBP) is a multifunctional protein required, amongst other things, for DNA replication and transcription control. It binds to single- and double-stranded DNA, as well as to RNA, in a sequence-independent manner. Like other single-stranded DNA binding proteins, it binds ssDNA cooperatively. We report the crystal structure, at 2.6 ANG resolution, of the nucleic acid binding domain. This domain is active in DNA replication. The protein contains two zinc atoms in different, novel coordinations. The zinc atoms appear to be required for the stability of the protein fold rather than being involved in direct contacts with the DNA. The crystal structure shows that the protein contains a 17 amino acid C-terminal extension which hooks onto a second molecule, thereby forming a protein chain. Deletion of this C-terminal arm reduces cooperatively in DNA binding, suggesting a hook-on model for cooperativity. Based on this structural work and mutant studies, we propose that DBP forms a protein core around which the single-stranded DNA winds.