Genetic stability of protein expression systems in yeast
Bussineau, C.M.; Shuster, J.R.
Developments in Biological Standardization 83: 13-19
1994
ISSN/ISBN: 0301-5149 PMID: 7883086 Document Number: 422649
For the expression of recombinant proteins in yeast, genetic stability is monitored using a combination of standard microbiology and nucleic acid testing procedures. Process consistency during the cell amplification and product expression phases of fermentation are also reliable indicators of stability. The potential for instability arising from point mutation, gene conversion or recombination has been shown to occur at a low frequency and does not generally affect protein product quality.