Gene therapy of cancer: use of IL-2 gene transfer and kinetics of local T and NK cell subsets
Bubeník, J.; Zeuthen, J.; Bubeníková, D.; Símová, J.; Jandlová, T.
Anticancer Research 13(5a): 1457-1460
1993
ISSN/ISBN: 0250-7005 PMID: 8239520 Document Number: 422276
Experiments were designed to compare the efficacy of recombinant IL-2 immunotherapy and IL-2 gene therapy of i.p. growing murine plasmacytoma X63-Ag8.653. The kinetics of peritoneal exudate mononuclear cells were monitored during the progression and gene therapy of the plasmacytoma, using cytofluorometric analysis and monoclonal antibodies against T and NK cell subsets. It has been found that the percentage of mice protected against plasmacytoma transplants was higher in mice treated by transfer of genetically manipulated IL-2-producing plasmacytoma cells as compared to the mice repeatedly injected with recombinant IL-2. Intraperitoneal inoculation of the X63-Ag8.653 plasmacytoma led in most of the inoculated mice to an increased percentage of NK+, ASGM-1+, Thy 1.2+, CD3+ and TCR-alpha-beta+ cells in the peritoneal fluid. The presence of macroscopically detectable i.p. tumours was accompanied by a higher increase in the percentage of NK+ and TCR-gamma-delta+ cells. Local IL-2 gene therapy of the plasmacytoma either prevented or diminished an increase in the percentage of CD3+, Thy 1.2+ and TCR-alpha-beta+ lymphocytes.