Nitric oxide complements prostacyclin in the regulation of endothelial thromboresistance under flow conditions
Korbut, R.; Ocetkiewicz, A.; Gryglewski, R.J.
Methods and Findings in Experimental and Clinical Pharmacology 15(3): 179-181
1993
ISSN/ISBN: 0379-0355 PMID: 8332034 Document Number: 421280
Using a novel method of measuring thrombogenesis on the surface of rabbit aorta endothelium superfused with whole blood, it was demonstrated that the thrombogenic properties of endothelium are potentiated by pretreatment with inhibitors of nitric oxide (NO) synthase (NG-monomethyl-l-arginine, meArg, 100 mcM) or cyclooxygenase (aspirin, ASA, 60 mcM). Since MeArg is less effective than aspirin and moreover does not influence thrombogenesis after previous inhibition of the synthesis of PGI-2, it is concluded that the generation of NO by vascular endothelium complements PGI-2 in its antithrombotic activity but NO may be ineffective when the generation of PGI-2 has been impaired.