Glycerol-loading test in experimental acute renal failure using 31P magnetic resonance spectroscopy of the kidney
Ishikawa, I.; Shikura, N.; Ishii, H.; Fukuda, Y.
Nihon Jinzo Gakkai Shi 35(3): 257-263
1993
ISSN/ISBN: 0385-2385 PMID: 8501855 Document Number: 412426
We observed that administration of exogenous glycerol reduces beta-ATP level and increases glycerophosphate level using magnetic resonance spectroscopy (MRS) of the kidney. A new "glycerol-loading test" for use with MRS in rats was developed. Sprague-Dawley rats were anesthetized and the left kidney was exposed and placed on surface coil. 31P-MRS (109.25MHz) of the kidney was performed using 10 microseconds, 90' pulse width and 2.308 sec repetition time with an accumulation of 200 times. MRS was performed 24 hours after induction of acute renal failure in each model of acute renal failure. Glycerol-loading test was performed by the intravenous infusion of 10% glycerol through jugular vein over a 60 min period. MRS was recorded every 10 min during glycerol infusion and for 60 min after cessation of infusion. In normal rats, beta-ATP level in the kidney was decreased to 52.0% of the pre-loading value at 55 min glycerol infusion and recovered to 66.7% 55 min after glycerol infusion was stopped. In the cisplatinum model, the reduction of beta-ATP and the increase of glycerophosphate levels following glycerol-loading was similar to that in normal rats; however, the recovery of beta-ATP level after infusion was stopped was weaker. Rats treated with glycerol and HgCl2 showed rather severe acute renal failure, but the beta-ATP level at 55 min glycerol infusion was 87.3% and 92.4%, respectively, showing difference from that in normal rats. No uptake of glycerol was observed 3 hours after 45 min pedicle clamping.