Experimental studies on in vitro maturation of porcine oocytes. I. Effects of medium, energy source and protein source on in vitro maturation rate
Eroglu, A.
Berliner und Münchener Tierärztliche Wochenschrift 106(5): 153-157
1993
ISSN/ISBN: 0005-9366 PMID: 8512548 Document Number: 411609
Effects of culture medium, energy and protein source on in vitro maturation of cumulus-intact porcine oocytes were examined. The bicarbonate buffered Whitten's medium was superior to the PB1 medium buffered with phosphate salts in supporting in vitro maturation. However, modification of energy source of the PB1 medium by reducing Na-pyruvate concentration and adding lactate salts gave also high maturation rate. The supplementation of Whitten's medium with 1 g and 3 g BSA/l as a protein source increased incidence of maturation and improved handling of oocytes. There was no significant difference in the ability of low concentration (1 g/l) and high concentration (3 g/l) of BSA in culture medium to support maturation.