Shortcomings of some currently available DNA probes for malaria detection
Ramasamy, R.; Ranasinghe, C.
Indian Journal of Medical Research 97: 108-111
1993
ISSN/ISBN: 0019-5340 PMID: 8406631 Document Number: 411478
A non-radioactive DNA prove based-method for detecting malaria will greatly aid epidemiological studies. Using putative Plasmodium falciparum and P. vivax-specific 18S ribosomal RNA directed oligonucleotides, different enzymatic and chemiluminescent detection methods were attempted without success. The sensitivity of the corresponding 32P labelled probes was found to be inadequate. A published procedure based on chemiluminescent detection of repetitive DNA sequences of P. falciparum was found to be adequately sensitive but lacking in specificity.
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