A micro-radioimmunoassay for the measurement of intracellular cAMP
Berg, W.; Hornbeck, P.V.
Biotechniques 15(1): 56-59
1993
ISSN/ISBN: 0736-6205 PMID: 7689853 Document Number: 409603
We have developed a rapid and sensitive micro-radioimmunoassay for the detection of cellular cAMP. The assay employs a simple method for preparing cellular lysates that requires as few as 500 cells per data point. Cellular lysates are incubated with cAMP-specific antibody complexes and (125I)cAMP in 96-well plates, harvested onto glass fiber filters and analyzed using a commercial autoradiograph imaging system. This method provides significant savings in reagents and may be particularly useful in situations where only a limited number of cells are available.