ATP and UTP increase secretion of bronchial inhibitor by human tracheal gland cells in culture
Merten, M.D.; Breittmayer, J.P.; Figarella, C.; Frelin, C.
American Journal of Physiology 265(5 Pt 1): L479-L484
1993
ISSN/ISBN: 0002-9513 PMID: 8238535 Document Number: 408655
The effects of ATP and UTP on intracellular Ca-2+ levels and on the secretion of the bronchial inhibitor and high-molecular-weight glycoproteins were studied in cultures of human bronchotracheal gland cells. ATP, adenosine 5'-O-(3-thiotriphosphate) (ATP-gamma-S), and UTP increased intracellular Ca-2+ levels in a manner that was partially dependent on the presence of extracellular Ca-2+. Other nucleotides (ADP, alpha,beta-methylene ATP, beta,gamma-methylene ATP, and 2-methylthio ATP) and adenosine were ineffective, thus suggesting the presence of a "nucleotide" receptor specific for ATP and UTP. At concentrations similar to those that raised intracellular Ca-2+ concentration, ATP, UTP, and ATP-gamma-S stimulate the secretion of the bronchial inhibitor. ATP and UTP also increase the production of sulfated high-molecular-weight glycoproteins. These results indicate the presence in human tracheal gland cells of a nucleotide receptor that mediates intracellular Ca-2+ mobilization and controls the secretion of macromolecules.