Homogeneous antigen receptor beta-chain genes in cloned CD4- CD8- alpha beta T suppressor cells
Schmidt-Wolf, I.G.; Liang, O.; Dejbakhsh-Jones, S.; Wang, H.; Cheng, L.; Holm, B.; Bell, R.; Strober, S.
Journal of Immunology 151(10): 5348-5353
1993
ISSN/ISBN: 0022-1767 PMID: 8228230 Document Number: 408649
The rearrangements of beta-chain genes of the T cell Ag receptor were examined in 12 CD4- CD8- alpha beta + T cell lines derived from the spleen or thymus of neonatal or adult BALB/c mice. Eleven of the lines were cloned and established from six independent cloning procedures from different mice. Five cloned lines used V beta 9, four cloned lines used V beta 15, and two cloned lines used V beta 7. Nucleotide sequencing of the beta-chain genes showed that clones that used a given V beta were identically rearranged even when they were derived from independent cloning procedures. In the case of V beta 7 and V beta 15 all nucleotides in the V-D-J joining region were in the germ line configuration without N region additions. Rearrangements of the V beta 7, V beta 9, and V beta 15 genes were functional. Each V beta 15 clone also had a homogeneous rearrangement of the V beta 13 gene, which was nonfunctional. The predicted amino acid sequence of the joining regions of the V beta 7, V beta 9, and V beta 15 rearrangements showed homology in four of seven amino acids in the peptide contact region.