Nitric oxide donors as generators and scavengers of superoxide anions
Robak, J.; Gryglewski, R.J.
Polish Journal of Pharmacology 45(1): 51-58
1993
ISSN/ISBN: 1230-6002 PMID: 8401759 Document Number: 406550
Three classical direct nitric oxide (NO) donors, 3-morpholine-sydnonimine (SIN-1), S-nitroso-N-acetyl-d,l-penicillamine (SNAP) and sodium nitroprusside (NaNP) as well as two indirect NO donors, molsidomine (MSL) and glyceryl trinitrate (GTN) were studied for their potencies to generate O-2-, to scavenge O-2-, to consume molecular oxygen and to inhibit lipid oxidation. Out of five NO donors only those which were spontaneous releasers of NO at physiological pH were also scavengers of O-2- which has been generated by xanthine : xanthine oxidase system (SIN-1 IC-50 19 mu-M, SNAP IC-50 416 mu-M) and inhibitors of the Fe-3+ and ascorbate stimulated oxidation of rat liver lipids (SIN-1 IC-50 76 mu-M, SNAP IC-50 12 mu-M). Only SIN-1 at high concentrations of 300-5000 mu-M generated O-2- as detected by a SOD inhibitable reduction of nitroblue tetrazolium. None of the in vitro studied activities were exerted by NaNP, MLS and GTN.