Methods of detection of anti-cathepsin G autoantibodies in human
Lesavre, P.; Nusbaum, P.; Halbwachs-Mecarelli, L.
Advances in Experimental Medicine and Biology 336: 257-261
1993
ISSN/ISBN: 0065-2598 PMID: 8296615 Document Number: 406449
Anti-cathepsin G antibodies have been detected by using three different methods. i) Binding to azurophilic granules constituents after separation of purified alpha-granules on Matrex gel Orange A chromatography according to Kao. ii) Binding to azurophilic granules freezed and thawed after coating on ELISA plates. iii) Binding to purified cathepsin G in ELISA assay. Anti-cathepsin G antibodies were observed patient's sera with ulcerative colitis, primary sclerosing cholangitis, primary biliary cirrhosis and autoimmune hepatitis but not in controls or patients with chronic viral hepatitis or vasculitis.