Antitumor activity and tumor necrosis factor production of Phytolacca acinosa polysaccharides I in mice
Zhang, J.P.; Qian, D.H.
Zhongguo Yao Li Xue Bao 14(6): 542-545
1993
ISSN/ISBN: 0253-9756 PMID: 8010055 Document Number: 406055
The antitumor activities of Phytolacca acinosa polysaccharides I (PAP-I) and its effects on the induction of tumor necrosis factor (TNF) and immunological cytotoxicity of peritoneal macrophages were studied. PAP-I was given up 5-20 mg cntdot kg-1 cntdot d-1 times 7 d to ICR mice as priming agent with subsequent lipopolysaccharides (10 mu-g/mouse) iv for TNF production. TNF activity was measured by crystal violet staining assay using L929 cells. PAP-I showed priming activity for TNF production with hepto-splenic hyperplasia in a dose-dependent manner. The peritoneal macrophages treated with PAP-I 10 and 20 mg cntdot kg-1 showed 67 and 74%, respectively, cytotoxicity (the control 34% cytotoxicity) against Meth A cells at effector: target = 40:1. PAP-I 10 and 20 mg cntdot kg prolonged the survival time of mice bearing ascites Meth A tumor from 21 +- 4 to 32 +- 10 and 38 +- 8 d and inhibited the solid Meth A tumor growth with intubation rate of 28.5 and 55.7%, respectively. These results suggested that the antitumor activities of PAP-I were based on the activation of macrophages and induction of TNF.