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Rat pancreas kinesin: identification and potential binding to microtubules

Malekzadeh-Hemmat, K.; Gendry, P.; Launay, J.F.

Cellular and Molecular Biology 39(3): 279-285

1993

ISSN/ISBN: 0145-5680
PMID: 8334381
Document Number: 405593
We have demonstrated the presence of kinesin in the secretory pancreatic tissue using SDS-PAGE, immunoblot and immunoelectron microscopy techniques. Polyclonal antibodies were raised against the rat brain kinesin heavy chain and affinity-purified. Immunoblot studies showed that these antibodies were bound to a 116 kDa protein found in rat pancreas crude extracts and in partially purified kinesin fractions. Kinesin identification was also performed by a cosedimentation procedure based on its strong binding to microtubules in the presence of sodium fluoride. The microtubule-kinesin complex was observed by immunoelectron microscopy gold staining. The reversible association of kinesin with microtubules was generated by MgATP.

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