Altered p53 gene structure and expression in human epithelial cells after exposure to nickel
Maehle, L.; Metcalf, R.A.; Ryberg, D.; Bennett, W.P.; Harris, C.C.; Haugen, A.
Cancer Research 52(1): 218-221
1992
ISSN/ISBN: 0008-5472 PMID: 1727381 Document Number: 400631
The carcinogenicity of certain nickel compounds is well known. We have previously shown that human kidney epithelial cells were immortalized by treatment with Ni(II) and in cooperation with the v-Ha-ras oncogene transformed the cells to acquire tumorigenicity in athymic nude mice. Immunocytochemistry and sequence analysis of DNA from the nickel-immortalized cells revealed abnormal p53 expression and a T.fwdarw.C transition mutation in codon 238. These data are consistent with the hypothesis that Ni(II)-induced mutation in the p53 gene can be involved in the escape from senescence of kidney epithelial cells.