Influence of calcium removal from bath solution on isolated rat duodenum contractions
Süer, C.; Erenmemisoglu, A.; Temocin, S.; Akar, S.; Beydagi, H.; Taspolat, I.
Indian Journal of Physiology and Pharmacology 36(3): 181-184
1992
ISSN/ISBN: 0019-5499 PMID: 1473848 Document Number: 399242
In this study whether extracellular Ca++ is essential to produce an increase of tension of isolated rat duodenum by ACh, 5-HT, AVP and KCl-, was examined. KCl- and AVP-evoked contractions were almost totally prevented by Ca++ removal from bath solution. The increase of tension of isolated duodenum caused by ACh or 5-HT was totally prevented after adding nifedipine, a Ca++ channel blocker, into Ca free solution. Our results suggest that ACh or 5-HT utilizes intracellular as well as extracellular sources of Ca++ to produce contraction in rat duodenum, whereas AVP- or KCl evoked contraction was mainly due to influx of Ca from extracellular sources.