A new deficiency mapping technique using the SOFI detector
Thérond, P.; Mastrippolito, R.; Tricoire, H.
Biotechniques 12(2): 252-257
1992
ISSN/ISBN: 0736-6205 PMID: 1616719 Document Number: 398948
We present a new technique for chromosomal deficiency mapping that takes advantage of the ability of the SOFI detector to provide fast quantitative data of very weak signals. With this new strategy, in contrast to the time-consuming traditional method, all the clones corresponding to a given genomic region may be mapped for their inclusion inside a deletion with only two hybridizations, independent of the size of the genomic region to be analyzed.
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