UV absorption complicates PCR decontamination
Frothingham, R.; Blitchington, R.B.; Lee, D.H.; Greene, R.C.; Wilson, K.H.
Biotechniques 13(2): 208-210
1992
ISSN/ISBN: 0736-6205 PMID: 1389153 Document Number: 397430
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
Document emailed within 1 workday
Related Documents
Haddad, P. 1999: PHARMAC complicates treatment of glaucoma New Zealand Medical Journal 112(1099): 434Augustine, J.J. 2006: Cold, wet and hurt. Hypothermia complicates trauma when a hunter falls fom a tree Emergency Medical Services 35(12): 36; 38
Keilin, D.; Hartree, E.F. 1949: Effect of low temperature on the absorption spectra of haemoproteins; with observations on the absorption spectrum of oxygen Nature 164(4163): 254-259
Gamot, A.; Guisez, N.; Mulleman, J.; Traisnel, M. 1971: Absorption capacity of active carbons and their pharmaceutic forms. 3. Interpretation of experimental curves of absorption Journal de Pharmacie de Belgique 26(5): 548-554
Pütter, J. 1970: Studies on absorption of salicylates. 2. Percutaneous absorption of salicylic acid 2'-hydroxyethyl ester following topic administration Arzneimittel-Forschung 20(11): 1721-1723
Buraczewska, L. 1981: Absorption of amino acids in different parts of the small intestine in growing pigs. III. Absorption of constituents of protein hydrolysates Acta Physiologica Polonica 32(5): 569-584
Tencova, A.; Kiszeleva, G.; Selmeczi, B.; Kedvessy, G.; Nagy, G. 1984: Technological processes and the influence of adjuvants on the dissolution rate and absorption of some ingredients from tablets. V. Release and absorption capacity of sulfadimidine Acta Pharmaceutica Hungarica 54(6): 245-248
Nakahashi, N.; Okamoto, S.; Hashimoto, C.; Shimizu, T.; Taguchi, K. 1971: Comparative evaluation of quantitative determination of lead in the body by atomic absorption sectrophotometry. the direct measurement using a long absorption tube Rinsho Byori. Japanese Journal of Clinical Pathology 19(Suppl): 185
Mahalanabis, D.; Patra, F.C. 1983: In search of a super oral rehydration solution: can optimum use of organic solute-mediated sodium absorption lead to the development of an absorption promoting drug? Journal of Diarrhoeal Diseases Research 1(2): 76-81
Okumura, T.; Suzuki, K. 2001: Gut decontamination--3--Gastrolavage Chudoku Kenkyu: Chudoku Kenkyukai Jun Kikanshi 14(2): 133-139
Vidon, N.; Hecketsweiler, P.; Cortot, A.; Bernier, J.J. 1977: A study of the absorption of an elemental diet administered by continuous jejunal perfusion: the effect of the rate of perfusion and the concentration upon the absorption of the nutrients Gastroenterologie Clinique et Biologique 1(3): 257-266
Fujita, M.; Tomo, N.; Abe, M.; Hosoi, K.; Sató, T. 1975: Experimental studies on absorption, distribution and excretion of a new antibiotic, fosfomycin. II. Absorption of oral preparations of fosfomycin calcium salt in dogs Japanese Journal of Antibiotics 28(3): 314-319
Esposito, G.; Zoppi, S. 1988: Intestinal absorption. II--Absorption of carbohydrates Il Farmaco; Edizione Pratica 43(7-8): 237-258
Grouzoulon, G. 1972: Intestinal absorption of hexoses. 3. Effect of a fructose enriched ration upon intestinal absorption of this carbohydrate Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 166(4): 639-642
Guiot, H.F. 1978: Partial antibiotic decontamination Pathologie-Biologie 26(1): 61
Walker, R.I.; MacVittie, T.J.; Sinha, B.L.; Ewald, P.E.; Egan, J.E.; McClung, G.L. 1978: Antibiotic decontamination of the dog and its consequences Laboratory Animal Science 28(1): 55-61
Lekkerkerker, J.F.; van Woudenberg, F.; Beekhuis, H.; Doorenbos, H. 1971: Enhancement of calcium absorption in hypothyroidism. Observations with a new method measuring calcium absorption Israel Journal of Medical Sciences 7(3): 399-400
Centi, R.; Bisiani, M.; Salicone, A.; Zaffiri, O. 1971: Decontamination of anesthesia and resuscitation equipment Minerva Anestesiologica 37(8): 340-345
Mĕrka, V.; Jebavý, L. 1991: Experience with the selective decontamination of the intestines Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 2: 30-32
Perry, H.; Shannon, M. 1996: Emergency department gastrointestinal decontamination Pediatric Annals 25(1): 19-26