P53 mutations in formaldehyde-induced nasal squamous cell carcinomas in rats
Recio, L.; Sisk, S.; Pluta, L.; Bermudez, E.; Gross, E.A.; Chen, Z.; Morgan, K.; Walker, C.
Cancer Research 52(21): 6113-6116
1992
ISSN/ISBN: 0008-5472 PMID: 1394239 Document Number: 394686
Formaldehyde induces squamous cell carcinomas in the nasal passages of rats following chronic inhalation exposure at concentrations of gtoreq 10 ppm. We have examined the complementary DNA of the tumor suppressor gene p53 from 11 primary formaldehyde-induced tumors for mutation using DNA sequence analysis. A polymerase chain reaction-amplified fragment of the rat p53 complementary DNA containing the evolutionarily conserved regions II-V was directly sequenced from each tumor. Point mutations in the p53 complementary DNA sequence were found in 5 of 11 of the tumors analyzed. These data demonstrate p53 point mutations in formaldehyde-induced squamous cell carcinomas and indicate a common alteration in certain rat and human squamous cell carcinomas of the respiratory tract.