A comparative study of the expediency of using different chemical methods for determining protein in allergen preparations
Tsvetkova, N.V.; Shmygleva, V.I.; Diakiv, V.V.; Borisenko, I.V.; Kuznetsova, E.M.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 1: 40-43
1992
ISSN/ISBN: 0372-9311 PMID: 1414105 Document Number: 393102
Parallel use of some known chemical procedures for measurements of protein in commercial forms of allergens revealed considerable variations in the results, which was due to the nature of the methods used and to admixtures of different substances in the preparations under study. The conclusion on the necessity of using at least two methods of protein determination for the standardization of allergens was made. Preference was given to Bradford's method. As the standardization of allergens manufactured in the USSR is carried out on the basis of the quantitative determination of protein (in PNU), this problem is of not only theoretical, but also practical importance.