Expression of EGF receptors in cell lines derived from female genital cancers and enhancement of the therapeutic effect of anticancer drugs by EGF
Ma, J.; Suzuki, M.; Takada, M.
Nihon Sanka Fujinka Gakkai Zasshi 44(1): 49-54
1992
ISSN/ISBN: 0300-9165 PMID: 1541862 Document Number: 392596
Manifestation of EGF receptors and enhancement of an anticancer agent by EGF were studied in cultured cells derived from female genital cancers. The numbers of EGF-receptors of SKG-3a, RMUG-s, HUOA, A-431 and HEC-1 were 1.22 .times. 104, 6.94 .times. 104, 2.75 .times. 104, 5.25 .times. 105 and 0.92 .times. 104 sites per cell respectively. The values for the dissociation constant (Kd) of RMUG-s, SKG-3a, HUOA and HEC-1 were 340pM, 477pM, 989pM and 2,187pM, respectively. All cell lines were stimulated by EGF at low concentrations and inhibited at higher concentrations. The growth stimulation rates for SKG-3a, HEC-1, HUOA and RMUG-s in the presence of 0.01nM EGF at 48 hours were 8, 18, 21.1 and 3.7%, respectively. The growth inhibition rates for SKG-2a, A-431, HEC-1, HUOA and RMUG-s in the presence of 1.0nM EGF at 48 hours were 32, 28, 25.8, 11.2 and 6.8%, respectively. The antitumor effect of CDDP was enhanced by the presence of EGF at both a concentration of 0.01 nM (except for A-431) and a concentration of 1.0nM, in all cell lines. EGF receptors of RMUG-s and HUOA were decreased by CDDP. The values for RMUG-s and HUOA were 1.71 .times. 104, 0.52 .times. 104 (CDDP 0.4 .mu.g/ml) and 0.95 .times. 104, 0.29 .times. 104 sites per cell (CDDP 2.0 .mu.g/ml), respectively. The effect of CDDP on EGF receptors was not recognized in SKG-3a and HEC-1. In brief, EGF receptors were significantly expressed in cell lines derived from female genital cancers. In addition, EGF may have an action in augmenting the action of CDDP to suppress the proliferation of cancer cells.