IFN-gamma induces a phospholipase D dependent triphasic activation of protein kinase C in endothelial cells
Mattila, P.; Renkonen, R.
Biochemical and Biophysical Research Communications 189(3): 1732-1738
1992
ISSN/ISBN: 0006-291X PMID: 1482378 Document Number: 389502
The IFN-gamma linked PKC activation in endothelial cells was analysed. It was shown that IFN-gamma activates PKC in three transient and separate cycles within the first 60 minutes after IFN-gamma stimulation. Before each PKC activation there was an increase in DAG level. IP-3, phosphocholine and choline productions were measured to determine the origin of DAG. Neither of the PLC products, IP-3 or phosphocholine, were released after IFN-gamma stimulation. On the other hand the PLD products choline and PA were released before all the three activation cycles of PKC.