Direct radioactive labeling of unpurified PCR products using Klenow fragment
McDaniel, T.K.; Huang, Y.; Yin, J.; Needleman, S.W.; Meltzer, S.J.
Biotechniques 11(2): 164; 166
1991
ISSN/ISBN: 0736-6205 PMID: 1931010 Document Number: 386511
We describe a method for rapid radioactive labeling of PCR product. The method, employing the Klenow fragment of DNA polymerase I, consumes little product, requires no product purification and takes under 30 minutes.
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