Top Abstract Purchase Related
Home
>
Node
>
Subnode

Segments of 18S rRNA, located in the area of the mRNA-binding center of ribosomes from human placenta

Graĭfer, D.M.; Zenkova, M.A.; Malygin, A.A.; Mamaev, S.V.; Mundus, D.A.; Karpova, G.G.

Bioorganicheskaia Khimiia 17(1): 88-98

1991

ISSN/ISBN: 0132-3423
PMID: 2064626
Document Number: 382528
The affinity labelling of human placenta 80S ribosomes by 4-(N-2-chloroethyl-N-methylamino)benzyl-5'-phosphoramide of hexauridylate has been studied. This mRNA analogue has normal coding properties because its binding to placenta ribosomes significantly increases in the presence of the cognate tRNA(Phe). Incubation of the mRNA analogue in the complex with ribosomes and Phe-tRNAPhe) leads to its covalent attachment exclusively to the small subunit (mainly to 18S rRNA). The reaction site has been shown by hybridisation experiments to be located within positions 975-1055 of 18S rRNA. The identified fragment is located in a highly conserved part of the small subunit rRNA domain II.

Document emailed within 1 workday
Secure & encrypted payments

Graĭfer, D.M.; Zenkova, M.A.; Karpova, G.G.; Malygin, A.A.; Matasova, N.B. 1990: Characteristics of nonenzymatic binding of oligoribonucleotides-- analogs of mRNA and Phe-tRNA Phe with 80S ribosomes from human placenta Molekuliarnaia Biologiia 24(4): 1076-1083
Budker, V.G.; Grineva, N.I.; Karpova, G.G.; Kobets, N.D.; Maev, S.P. 1977: Affinity modification of Escherichia coli ribosomes in the region of the mRNA-binding center by a heptauridylate analog bearing a chemically active group at the 3' end Molekuliarnaia Biologiia 12(3): 539-545
Babkina, G.T.; Vladimirov, S.N.; Dryga, S.A.; Zenkova, M.A.; Karpova, G.G. 1988: MRNA-binding site of ribosomes at different stages of translation. II. Affinity modification of Escherichia coli ribosomes bya benzylidene derivative of AUGU6 in pre- and post-translation complexes Molekuliarnaia Biologiia 22(2): 357-361
Urlaub, H.; Kruft, V.; Bischof, O.; Müller, E.C.; Wittmann-Liebold, B. 1995: Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies EMBO Journal 14(18): 4578-4588
Kostopoulou, O.N.; Papadopoulos, G.; Kouvela, E.C.; Kalpaxis, D.L. 2013: Clindamycin binding to ribosomes revisited: foot printing and computational detection of two binding sites within the peptidyl transferase center Die Pharmazie 68(7): 616-621
Mann, K.K.; André, S.; Gabius, H.J.; Sharp, J.G. 1994: Phenotype-associated lectin-binding profiles of normal and transformed blood cells: a comparative analysis of mannose- and galactose-binding lectins from plants and human serum/placenta European Journal of Cell Biology 65(1): 145-151
Korobkova, E.S.; Panchenko, L.P.; Shalamai, A.S.; Skripal, I.G. 1995: The ability of the oligodeoxyribonucleotides complementary to the 3'-terminal segment of 16s-rRNA in Mollicutes to suppress translation in their ribosomes in an in-vitro system Mikrobiolohichnyi Zhurnal 57(3): 30-36
Okubo, A.; Sameshima, M.; Unoki, K.; Uehara, F. 1995: The ultrastructural study of ribosomes in photoreceptor inner segments of the pcd cerebellar mutant mouse Japanese Journal of Ophthalmology 39(2): 152-161
Yan, D.; Gu, X.; Jiang, S.; Wang, Y. 2015: MRNA-binding protein Human-antigen R regulates α-SMA expression in human bronchia smooth muscle cells Zhonghua Yi Xue Za Zhi 95(38): 3147-3149
Kamath, S.G.; Kelley, L.K.; Friedman, A.F.; Smith, C.H. 1992: Transport and binding in calcium uptake by microvillous membrane of human placenta American Journal of Physiology 262(3 Pt 1): C789-C794
Karpyza, E.I.; Ermolenko, M.N.; Sviridov, O.V. 1990: Characteristics of specific binding of thyroid hormones with plasma membranes of syncytiotrophoblast of human placenta Voprosy Meditsinskoi Khimii 36(3): 45-48
Mordukhovich, A.S.; Reznik, F.I. 1974: Clinical and immunomorphological data in premature detachment of normally located placenta Akusherstvo i Ginekologiia 7: 34-36
Nierhaus, K.H.; Montejo, V.; Nierhaus, D.; Diertich, S.; Schrandt, I. 1974: Proteins at the peptidyltransferase center from E. coli ribosomes Acta Biologica et Medica Germanica 33(5-6): 613-619
Taniguchi, S.; Watanabe, T.; Nakao, A.; Seki, G.; Uwatoko, S.; Kurokawa, K. 1994: Detection and quantitation of EP3 prostaglandin E2 receptor mRNA along mouse nephron segments by RT-PCR American Journal of Physiology 266(5 Pt 1): C1453-C1458
Miura, K.; Tsuda, S.; Kakuchi, J.; Harada, F.; Ueda, T. 1983: Chemical modification of adenine residues in mouse 5S rRNA with monoperphthalate: the secondary structure of 5S rRNA Nucleic Acids Symposium Series 12: 161-163
Stahl, J.; Karpova, G.G. 1985: Investigations on the messenger RNA binding site of eukaryotic ribosomes by using reactive oligo(U) derivatives Biomedica Biochimica Acta 44(7-8): 1057-1064
Hui, A.; Jhurani, P.; de Boer, H.A. 1987: Directing ribosomes to a single mRNA species: a method to study ribosomal RNA mutations and their effects on translation of a single messenger in Escherichia coli Methods in Enzymology 153: 432-452
Roman, S.; Moore, J.S.; Darby, C.; Müller, S.; Hoover, R.G. 1988: Modulation of Ig gene expression by Ig binding factors. Suppression of alpha-H chain and lambda-2-L chain mRNA accumulation in MOPC-315 by IgA-binding factor Journal of Immunology 140(10): 3622-3630
Wang, Y.; Lin, Q.; Wang, X.; Lu, P.; Sheng, Z.; Ding, C.; Hong, Y.; Li, W. 2002: Expression of lethal gene mRNA on placenta villi in patients with spontaneous abortion Zhonghua Fu Chan Ke Za Zhi 37(9): 542-545
Kurek, E.; Grankowski, N.; Gasior, E. 1972: On the phosphorylation of Escherichia coli ribosomes. I. An in vivo labeling of ribosomes Acta Microbiologica Polonica. Series A: Microbiologia Generalis 4(4): 171-176