Potent collagenase inhibitors prevent interleukin-1-induced cartilage degradation in vitro
Nixon, J.S.; Bottomley, K.M.; Broadhurst, M.J.; Brown, P.A.; Johnson, W.H.; Lawton, G.; Marley, J.; Sedgwick, A.D.; Wilkinson, S.E.
International Journal of Tissue Reactions 13(5): 237-241
1991
ISSN/ISBN: 0250-0868 PMID: 1666894 Document Number: 380324
The matrix metalloproteinases (MMPs) collagenase, gelatinase and stromelysin, contribute to the destruction of articular cartilage which occurs during rheumatoid and osteoarthritis. Ro 31-4724, a substrate analogue containing a hydroxamic acid function, is a potent but non-selective inhibitor of all three MMPs (I50, collagenase = 10 nM), whereas Ro 31-7467, a phosphinic acid transition-state analogue, shows 14-fold and 12-fold selectivity for collagenase (I50 = 17 nM) over gelatinase and caseinase (stromelysin) respectively. The effects of these inhibitors on interleukin-1-induced bovine nasal cartilage degradation were examined. The hydroxamate Ro 31-4724 inhibits proteoglycan and collagen loss, whereas the phosphinic acid Ro 31-7467 selectively inhibits collagen breakdown in this model. This represents the first demonstration of potent and selective inhibition of IL1-induced cartilage degradation in vitro by MMP inhibitors. These results suggest that collagenase is responsible for collagen loss and that a different enzyme, possibly stromelysin, is responsible for proteoglycan degradation in this model.